| 摘要 |
PURPOSE:To enable obtaining of the subject gene in a large amount by containing one or more of specific antigenic parts of human centromere antigen recognizable with a specified antibody. CONSTITUTION:A cDNA library (A) is prepared from an mRNA obtained from Jurkat cell derived from human T cell. The resultant ingredient (A) is subsequently screened to provide a clone (C) capable of expressing a polypeptide having the activity of human centromere antigen (B). The obtained ingredient (C) is then subjected to treatment with a restriction enzyme to afford a DNA fragment (D) capable of coding a polypeptide containing one or more of antigen specific parts of the ingredient (B). The ingredient (D) is subsequently integrated into a desired vector for expression to provide a recombinant plasmid (E), which is then transduced into Escherichia coli, etc., to afford a transformant (F). The resultant transformant (F) is cultured to provide a culture (G), which is subsequently purified by column chromatography, etc., to produce human centromere antigenic polypeptide (H). The prepared ingredient (H), as necessary, is brought into contact with a specimen such as blood to measure the anti- centromere antibody activity according to an immunoassay, etc. |
