PRODUCING MONOCLONAL ANTIBODIES AGAINST HUMAN T-CELL RECEPTOR ELEMENTS USING RECOMBINANT DNA VECTORS

摘要

Recombinant DNA vectors which express chimeric T cell receptors are disclosed. These chimeric T cell receptors contain one human element, and the rest of the elements are all of the same, non-human animal species, such as a mouse. Of particular interest are chimeras where the human element is V alpha or V beta . The vectors are used to transfect cells which derive from te same non-human animal species as the non-human animal species of the chimera, and the resulting transfectants are used to produce monoclonal antibodies against the human element of the chimera.