| 摘要 |
2088776 9203542 PCTABS00011 A proteolytic factor is capable of cleaving .beta.-protein precursor at a site near the .beta.-protein N-terminus. Also, a method for treating Alzheimer's disease in a patient includes steps of reducing $(b)-protein precursor proteolysis outside the .beta.-protein domain at a site near the .beta.-protein N-terminus. Also, a method for purifying en enzyme from a sample includes steps of incubating the sample with a labelled substrate of the enzyme or with a labelled fragment of a substrate to which the enzyme binds, treating the sample with a crosslinking agent to crosslink any enzyme-substrate complexes in the sample, and recovering labelled complexes. Also, a method for diagnosis in a subject of a disease characterized by accumulation of amyloid includes determining the level, in a sample of tissue or body fluid from the subject, of an AD proteolytic factor. Also, a method for screening for an agent useful in treatment of a disease characterized by accumulation of amyloid includes steps of incubating an AD protease with a peptide having an amino acid sequence corresponding to the sequence spanning the .beta.-protein N-terminus in the presence of a candidate agent, and determining the degradation of the peptide.
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