发明名称 |
DNA IMMOBILIZED MICROSPHERE AND PURIFICATION OF DNA TRANSCRIPTION CONTROL FACTOR USING THE SAME MICROSPHERE |
摘要 |
PURPOSE:To obtain DNA immobilized microspheres capable of being purified from a specific protein readily and in high yield, comprising a specific DNA chain bonded to a carrier composed of a resin containing epoxy group on the surface by covalent bond to subject the epoxy group to ring opening. CONSTITUTION:DNA immobilized microspheres comprising a DNA chain having a base sequence to specifically bond a specific protein, bonded to a carrier composed of a resin containing epoxy group on the surface by subjecting the epoxy group to ring opening and not adsorbing protein except the base sequence part. An epoxy group-containing (meth)acrylic ester (co)polymer may be cited as the resin to form the carrier surface and polyglycidyl methacrylate prepared by soap-free emulsion polymerization using especially a water-soluble azo-based polymerizing agent is preferable. A DNA transcription control factor may be cited as the protein to be purified by using DNA immobilized microspheres. |
申请公布号 |
JPH0446193(A) |
申请公布日期 |
1992.02.17 |
申请号 |
JP19900148701 |
申请日期 |
1990.06.08 |
申请人 |
NIPPON ZEON CO LTD |
发明人 |
KAWAGUCHI HARUMA;INOMATA YUKIO;HANDA HIROSHI |
分类号 |
C12N15/09;C07K1/14;C07K1/16;C07K1/22;C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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