| 摘要 |
PURPOSE:To make it possible to obtain a clear DNA band by laminating two flat plates through a spacer in the longitudinal direction, and filling the gap between the flat plates with agarose gel. CONSTITUTION:Spacers A 4 are aligned in the longitudinal direction at both end parts of a glass flat plates A 2 in the direction of the width so that the width of agarose gel is about 180 mm. Spacers B 5 are aligned in the direction of the width of the flat plate A 2 so that the spacer B 5 is in contact with the end parts of both spacers A 4. A glass flat plate B 3 is laminated. A glass flat plate C 6 is laminated so that the surface from which a comb 61 is protruding is in contact with the agarose gel. A sample 12 is mounted on a well 91 and migrated at the voltage of about 4.8 V/cm. The sample 12 is submerged into the agarose gel 9. The sample is further migrated at the voltage of about 3.26 V/cm. Then, the flat plate B 3 and the flat plate C6 are separated and dyed. A gel electrophoresis member 1 is mounted on a light-transmitting-type ultraviolet-ray emitting device, and the clear DNA band can be observed. |
