| 摘要 |
<p>PURPOSE:To culture a large amount of adventitious embryo, by culturing inducing cells in a high-density state to give spherical embryo and heart shaped embryo in a mixed state and culturing the mixture of the spherical embryo and heart shaped embryo in a low-density state. CONSTITUTION:Culture cells of dicotyledon are cultured in a medium for adventitious embryo differentiation in a high culture density, e.g. 2,000 cells/ml to give spherical embryo and heart shaped embryo in about 90% differentiation ratio, these embryos are cultured in a low culture density, e.g. about 150 embryos/ml to culture a large amount of adventitious embryos of dicotyledon. By using this method, synchronization can be attained up to the latter period of the differentiation, namely torpedo shaped embryo and moreover, the culture cells used can be differentiated in high frequency close to 80%. Further the number of days required for a culture period can be more shorted than ever. When the prepared torpedo shaped embryos are cultured under light irradiation to give young plants.</p> |
