METHOD FOR LABELING POLYNUCLEOTIDE SEQUENCES

摘要

A method for labeling a polynucleotide sequence to form a polynucleotide probe is disclosed. The method comprises forming a modified polynucleotide sequence wherein the modification comprises the cleavage of preselected bases to generate sugar aldehydes. The preselected bases are either quaternized nitrogen purines or uracil. A signalling moiety is attached to the modified polynucleotide sequence by means of the sugar aldehydes.