METHOD OF DETERMINING SOURCES OF ACETYL-COA UNDER NONSTEADY-STATE CONDITIONS

摘要

The present invention relates to a method of measuring the contribution of one or more exogenously administered <13>C-labeled substrates to acetyl-CoA. The measurement can be made in a tissue or cell using <13>C NMR without the constraint of metabolic or isotopic steady-state. Furthermore, the method permits the determination even when spectral lines are broad due to B0 inhomogeneity, thereby opening the way for substrate utilization studies in vivo. The method does not require many of the simplifying assumptions involved in <11>C or <14>C methods, and, since a stable isotope, <13>C, is used, a wide variety of compounds with complex labeling patterns may be synthesized and studied.