| 摘要 |
A method of reacting a linear mixed anhydride reaction product of a peptide and acetic anhydride with a reagent is disclosed. This invention provides reagents useful for the derivatization of prolyl containing peptides. The reagents of the invention comprise a class of compounds having a 3-amino-2-halo pyridine structure. Such compounds function as nucleophilic reagents with respect to acetic acid anhydride activated proline residues. Under appropriate conditions the amide nitrogen of the proline residue may displace the halogen of the reagent to provide a cleavable cyclic derivative. The reagents of the invention thus accommodate C-terminal sequencing of prolyl containing peptides. |
