METHOD FOR ISOLATION AND EXPRESSION OF GENE FOR CODING STREPTOKINASE AND NUCLEOTIDE SEQUENCE, RECOMBINANT DNA AND TRANSFORMED MICROORGANISM OBTAINED THEREFROM

摘要

The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms. The technical object which is pursued with the proposed solution is to produce the protein in commercially useful quantities, by the recombinant method, with the possibility of alternatively using different hosts to obtain a high-quality streptokinase capable of being used as a thrombolytic agent in the treatment of different disorders. The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase. The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.