RECOMBINANT PLASMID DNA PTRC TE-LEP ENCODING POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF POLYPEPTIDE WITH HUMAN LEPTIN PROPERTY

摘要

biotechnology, genetic engineering. SUBSTANCE: recombinant plasmid DNA comprises cDNA of processed form of human leptin, Escherichia coli trc-promoter and synthetic site as an enhancer of translation of bacteriophage T7 gene 10 which determining biosynthesis of polypeptide showing properties of human leptin. The strain Escherichia coli W3110/pTrcTE-Lep transformed with the prepared plasmid provides synthesis of this polypeptide at the expression level 43% of total cellular protein, not less, at concentration isopropyl-beta-D-thiogalactopyranoside as an inductor 0.05 mM. EFFECT: increased yield of polypeptide biosynthesis, simultaneous decrease of amount of inductor added. 2 cl, 5 dwg, 6 ex