Microbiological hydrolysis for the preparation of (exo,exo)-7-oxabicyclo(2.2.1) heptane-2,3, monoacyl ester dimethanol

摘要

Disclosed herein is a process for preparing a novel monoester of the formula in which the associated novel diester is hydrolyzed in the presence of one or more water-soluble enzymes or microorganisms capable of selectively hydrolyzing the -O-C(O)-R1 group, wherein the treatment is carried out in a biphasic solvent system comprising an aqueous phase having the enzymes or microorganisms and an organic phase immiscible in water having the diester. Also disclosed is a process for preparing [1S-[1 alpha , 2 alpha (Z),3 alpha ,4 alpha ]]-7-[3[[[[(1-oxoheptyl)amino]acetyl]-amino]methyl-7-oxabicyclo-[2.2.1 ]hept-2-yl]-5-heptenoic acid using this enzymatic/microbial process.