发明名称 |
ENZYME ELECTROCHEMICAL SENSOR ELECTRODE AND METHOD OF MAKING IT |
摘要 |
An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.
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申请公布号 |
US5082550(A) |
申请公布日期 |
1992.01.21 |
申请号 |
US19890448475 |
申请日期 |
1989.12.11 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF ENERGY |
发明人 |
RISHPON, JUDITH;ZAWODZINSKI, THOMAS A.;GOTTESFELD, SHIMSHON |
分类号 |
G01N27/327;C12Q1/00 |
主分类号 |
G01N27/327 |
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