| 摘要 |
PURPOSE:To effectively prepare a protein by reacting a thio-messenger RNA (SRNA) with ribosome, etc., the thio-messenger RNA being obtained by reacting a specific compound with RNA polymerase, etc. CONSTITUTION:A compound of formula I, II, III or IV (Y is oxygen atom, but at least one of four kinds of the compounds is sulfur atom), the template of DNA extracted from microoganism, etc., and T7 phase RNA polymerase are made to react with each other to prepare a thio messenger RNA (A) of formula [X is adenine, cytosine, guanine or uracil; Y is sulfur or oxygen atom; (m) is 0, 1 or 2; (n) is 50-1000; contains repeating units of formula IV (when X is one selected from adenine, cytosine, guanine and uracil, Y is sulfur atom)]. The temperature of the component A, ribosome, transfer RNA and an energy supply source such as ATP are compounded to prepare a synthetic reaction system (B). In the reaction system B, one kind or more of amino acids are allowed to react to form peptide bonds for the preparation of a protein.
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