| 摘要 |
An expression vector replicable in a mammalian cell includes a prepro-NGF encoding DNA sequence operably linked with regulatory DNA capable of effecting the expression of the prepro-NGF encoding DNA sequence in the cell. Also, a mammalian cell is transfected with such an expression vector. Also, a method for making recombinant human nerve growth factor includes transfecting a mammalian cell with such an expression vector, maintaining the cell in a culture medium, and isolating recombinant human nerve growth factor from the medium. Also, a composition includes recombinant human nerve growth factor made according to such a method combined with a pharmaceutically acceptable carrier.
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