发明名称 |
Novel amino acid substituted 4-aminophenazones for measuring enzyme activity |
摘要 |
Novel substrates which are amino acid 4-amino phenazones for detecting enzymes, particularly L-gamma-glutamyltransferase, are described. The amino acid gamma-glutamyl-4-aminophenazone reacts with the enzyme to produce 4-aminophenazone which is an amino dye intermediate. The amino dye intermediate is coupled with a second dye intermediate to form a chromogen. The 4-aminophenazone is particularly reacted with a phenolic naphtholic or aniline compound, particularly the phenolic compound 2-hydroxy-3,5-dichlorobenzenesulfonate, to form a red chromogen in the presence of an oxidizing agent, such as bilrubin oxidase.
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申请公布号 |
US5081259(A) |
申请公布日期 |
1992.01.14 |
申请号 |
US19900586995 |
申请日期 |
1990.09.24 |
申请人 |
ABBOTT LABORATORIES;BOARD OF GOVERNORS OF WAYNE STATE UNIVERSITY |
发明人 |
ARTISS, JOSEPH D.;BENSIE, JILL M.;ZAK, BENNIE;RADEN, DANIEL S. |
分类号 |
C07K5/02;C07K5/06;C07K5/072 |
主分类号 |
C07K5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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