ENZIMI FIBRINOLITICI AMMIDATI E LORO PRECURSORI, E PROCESSI PER LA LORO PREPARAZIONE.

摘要

Amidated fibrinolytic enzymes are provided which are -COOH-terminal amidated derivatives of human pro-urokinase. These derivatives have the general formula P-CONH2 (I) in which P represents the portion of human pro-urokinase, or a modification thereof, preceding the carboxy terminus. The modifications may be by way of deletion, insertion or substitution. A precursor of such derivatives has the formula P-Gly-Yn (I) wherein Y is any basic amino acid residue and n is 0 or an integer of 1-4. Processes for preparation of formula II via recombinant DNA techniques are detailed. Compounds of formula II provide precursors for in vitro or in vivo enzymatic amidation. Pharmaceutical compositions comprising compounds defined in formula (I) or (II) are provided for the treatment of acute myocardial infarction, pulmonary embolism or deep venous thrombosis.