| 摘要 |
<p>A protein sequencing method utilizing a composition of matter comprising a reagent polypeptide complex A-B-C-D wherein A is a polypeptide which contains an N-terminal amino acid unit B, C is an aromatic ring which contains a functional group which can react with and bind to the N-terminal amino acid of the polypeptide and can result in unit derivatization of the terminal amino acid and D comprises those moieties attached to the aromatic ring that contribute to the electrochemical activity of the derivatized N-terminal amino acid and are selected from those functional groups that do not interfere with the reactivity of the functional groups of the C moiety in a derivatization process. In its method form, the identity of an N-terminal amino acid is established through derivatization with said A-B-C-D reagent followed by chromatographic analysis and electrochemical detection.</p> |
