MULTILAYER ANALYTICAL ELEMENT FOR NON-ISOTOPIC ASSAY

摘要

A multilayer analytical element for non-isotopic assay of an analyte contained in an aqueous liquid sample, utilizing a competitive binding reaction to a protein between the analyte and a non-isotopically labeled analyte substance or analogue thereof, which comprises: a light-transmissive water-impermeable support; a sheet provided on the support, which comprises a continuous binder matrix containing the non-isotopically labeled analyte substance or analogue thereof; a porous reaction sheet provided on the binder matrix sheet, which contains a predetermined amount of the protein fixed therein, the protein being specifically bindable to the analyte; and a porous sharing sheet provided on the reaction sheet, in which the substantial separation of the bound non-isotopically labeled complex (B) from the free non-isotopically labeled analyte substance or analogue thereof (F) is accomplished by the presence of said porous sharing sheet. A slide enclosing the multi layer analytical element and a non-isotopic assay employing the multilayer analytical element are also disclosed.