Enhancing D-xylose and L-arabinose utilization in Zymomonas cells

摘要

Disrupting expression of a protein encoded by the region of the Zymomonas genome called ZMO0353 in the genomic sequence of the ZM4 strain was found to improve the use of xylose in a recombinant xylose utilizing Zymomonas cell. In addition, utilization of both xylose and arabinose was improved in a xylose and arabinose utilizing Zymomonas cell with this disruption, and increased ethanol production was achieved.

主权项

1. A recombinant Zymomonas mobilis cell that comprises D-xylose utilization pathway genes and that utilizes D-xylose as a carbon source, wherein the recombinant cell comprises at least one genetic modification of a genomic nucleic acid comprising a nucleic acid sequence having at least 95% sequence identity to the nucleotide sequence of SEQ ID NO: 1, wherein the at least one genetic modification disrupts expression of a functional enzyme encoded by the nucleic acid sequence, and wherein D-xylose utilization by the recombinant cell is increased as compared to D-xylose utilization in a cell that is identical except for lacking the genetic modification.