| 发明名称 | Human B1 cells and uses thereof | ||
| 摘要 | The present invention is directed to isolated populations of human natural immunoglobulin-producing B1 lymphocytes, wherein the B1 lymphocytes display surface biomarkers CD20, CD43 and CD27 and are either CD11b+ or GD11b−. The present invention is also directed to a method of isolating human natural immunoglobulin-producing B1 lymphocytes from a blood sample comprising isolating B lymphocytes from the sample that express surface biomarkers CD20, CD43 and CD27, and, optionally, CD11b. In addition, the present invention is directed to a methods for diagnosing a B1 cell disorder in a patient, determining the prognosis of a patient having a B1 cell disorder, and treating a patient having a B1 cell disorder. | ||
| 申请公布号 | US9371514(B2) | 申请公布日期 | 2016.06.21 |
| 申请号 | US201113825375 | 申请日期 | 2011.09.22 |
| 申请人 | The Feinstein Institute for Medical Research | 发明人 | Rothstein Thomas L.;Griffin Daniel O.;Holodick Nichol E. |
| 分类号 | C12N5/02;G01N33/50;C12N5/0781;A61K35/17;G01N33/564;G01N33/569;G01N33/574;A61K39/395;G01N33/68;A61K35/12 | 主分类号 | C12N5/02 |
| 代理机构 | Amster, Rothstein & Ebenstein LLP | 代理人 | Amster, Rothstein & Ebenstein LLP |
| 主权项 | 1. A method of isolating a population of human natural immunoglobulin-producing B1 lymphocytes from a human blood sample, wherein the B1 lymphocytes co-express surface biomarkers CD20 and CD43 and CD27, comprising isolating B lymphocytes from the sample that co-express surface biomarkers CD20 and CD43 and CD27 by contacting the sample with fluorescently labelled antibodies directed to CD20 and CD43 and CD27 and obtaining the fluorescently labelled B1 lymphocytes by fluorescence-activated cell sorting (FACS). | ||
| 地址 | Manhassett NY US | ||