EXPRESSION VECTORS, HOST CELLS COMPRISING SUCH VECTORS AND METHODS OF MAKING DESIRED COMPOUNDS USING SUCH CELLS

摘要

An expression vector having two structural genes that form a synthetic operon expressed under the control of a single regulatory sequence. The operon genes correspond to the structural component of naturally occurring genes whose expression is controlled by distinct separate regulatory sequences. The operon genes code for enzymes in a biosynthetic pathway for producing a desired compound, and at least one of those operon genes is feedback derepressed. The vector is used to transform host cells that are cultured to produce the desired product.