Disclosed herein is a culture bag which has a culture room formed by fusion-bonding a transparent sheet and at least one opening formed therein, said sheet being made of a resin composition composed of 100 parts by weight of vinyl chloride homopolymer or copolymer and 150-260 parts by weight of ethylene-n-butyl acrylate-carbon monoxide copolymer, and having a gas permeability of 600-3000 ml/m2?24hr?atm to oxygen and a gas permeability of 1000-30,000 ml/m2?24hr?atm to carbon dioxide.