Release and isolation of N-glycans.

摘要

The invention relates to a method for the release of N-glycan from a glycoconjugate and to isolation of such glycan. The invention provides a method which includes subjecting a glycoconjugate to the influence of a hydrazine reagent, said glycoconjugate being substantially salt-free, and controlling conditions under which the glycoconjugate is subjected to the influence of the hydrazine reagent so as to cause release of N-glycan from the glycoconjugate in such a way as to allow subsequent recovery of N-glycan in substantially unreduced and intact form and in a high yield. Released glycan may be isolated in accordance with the invention by use of a chromatographic material and, if required, performing an N-acetylation reaction and converting any acetohydrazone derivative to unreduced N-glycan. <IMAGE>