| 摘要 |
PURPOSE:To obtain a highly sensitive detecting method capable of applying an immunity inspecting method of a trace of component and enzyme determination method, by combining a photographic active material such as a spectral sensitization coloring matter or a fogging agent with silver halognide. CONSTITUTION:Water absorbing layer and photosensitive emulsion layer of silver halogenide are provided in order on a inspection sheet on a support body. The water absorbing layer is used for increasing the amount of water absorption and is held between the emulsion layer and support body and also, it is preferably to have nonsensitivity and swelling property. An inspection method by said inspection sheet is as follows; A synthetic substrate containing at least each one of construction singularly contacting with the enzyme to be measured or that of spectral sensitization coloring matter or fogging agent in the same molecule is used and either of the reaction product containing the spectral sensitization coloring matter construction or fogging agent construction produced by the enzyme reaction of the substrate and the enzyme to be measured or unreacted synthetic substrate, is brought contact with silver halgenide. Then, developing is carried out after exposure (spectral sensitization coloring matter) or as it is (fogging agent) and the amount of the enzyme is measured from the concentration of the coloring matter.
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