USE OF 1-(1-PYRROLIDINYLCARBONYL) PYRIDINIUM SALTS TO ATTACH COMPOUNDS TO CARBOXYLATED PARTICLES AND A KIT CONTAINING SAME

摘要

USE OF 1-(1-PYRROLIDINYLCARBONYL)PYRIDINIUM SALTS TO ATTACH COMPOUNDS TO CARBOXYLATED PARTICLES AND A KIT CONTAINING SAME Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl groups to polymeric particles having pendant carboxyl groups on the outer surfaces. Such reactive compounds include biologically reactive species, including enzymes, polypeptides and proteins. This attachment is carried out using specific carbamoylonium compounds, namely certain 1-(1-pyrrolidinylcarbonyl)pyridinium salts. These compounds react with the carboxyl groups on the particles to form intermediate reactive groups which then react with the amino or sulfhydryl groups to form a covalent linkage between particle and reactive compound. A kit comprises polymeric particles having carboxyl groups on the outer surfaces, and a 1-(1-pyrrolidinylcarbonyl)pyridinium salt.