| 摘要 |
PURPOSE:To improve a freezing preservation effect of an embryo without needing an icing device and to save a labor for a work to transplant to an embryogeny animal by mounting a substance stabilized by mixing an ice crystal inducing agent in a carrier to the mouth part of a freezing preserving container or sealing a mouth part with the stabilized substance during the icing operation. CONSTITUTION:The mouth of a preserving container is sealed with a substance in which an ice crystal inducing agent, e.g. silver iodide, having an icing function is mixed in a carrier consisting of polyvinyl alcohol (PVA) and other adhesive at -3.0 deg.C or less. Or, the icing is effected in a way that the ice crystal inducing agent and the adhesive are mixed together and a part of the inside making contact with a preserving liquid is coated with the mixture. Since the ice crystal inducing agent is stabilized for use, the ice crystal inducing agent is not moved in freezing preserving liquid, and an adverse influence is not exercised on an embryos when the freezing preserving container is sealed with a substance mixed with PVA powder for stabilization, there is no need to heat-seal the container, and when other adhesive is mixed for stabilization to mount the mixture on the inner wall of the container, heat seal is used in combination. Even when stabilization is effected by using PVA powder serving as a carrier and other adhesive, performance of the ice crystal inducing agent is entirely prevented from lowering. |
