| 摘要 |
Bulk fluid movement in a capillary column is achieved by creating electroendosmotic flow in a fluid passage outside the column, the fluid passage being joined to the column in a manner which causes the electroendosmotic flow to generate bulk flow in the column and yet permits an electric potential to be applied across the fluid passage and not the column. Small volume bulk fluid movement induced in this manner may be used to load sample into the column, whereas a larger induced volume may be used for flushing the column between runs. To alternate the bulk fluid movement with electrophoretic separations in the column, the column is arranged such that an electric potential can also be applied across the column without extending to the electroendosmotic fluid passage.
|
