PROCEDE DE DETECTION D'UNE SEQUENCE NUCLEOTIDIQUE SELON LA TECHNIQUE D'HYBRIDATION SANDWICH.

摘要

A method for detecting a single-stranded nucleotide sequence in a sample which is known or thought to contain it, using sandwich hybridization comprising the step of incubating the sample with a capture probe which is passively fixed to a solid support and with a detector probe which is labelled with a non-radioactive marker. The capture and detector probes can hybridize with two non-overlapping regions respectively of the target nucleotide sequence being sought. The incubation step is followed by a washing step for removing reagents which have not been fixed by hybridization. The method is characterized in that the capture probe contains 9-30 nucleotides and is fixed to said solid support which is made of a material based on a hydrophobic compound. The method can be used particularly for diagnosing infectious or genetic diseases and for cell typing.