METHOD OF PREPARING PLASMID HAVING CAPABILITIES OF EXPRESSING AND PROCESSING AFTER TRANSLATION OF RETROVIRUS GENE, PLASMID OBTAINED THEREBY AND PRODUCT OF EXPRESSION THEREOF

摘要

Three kinds of core protein coded for with a gag gene and three kinds of enzyme coded for with a pol gene are each separately mass-produced as a mature or active protein by inserting cDNA fragments containing at least protease genes among retrovirus genes into mass-expressed genes and directly gene expressing vectors to prepare a plasmid, expressing retrovirus genes therewith, and processing the product of expression per se with a protease present in said product. Alternatively, cDNA fragments containing a nef gene domain of HIV is inserted into mass-expressed genes and directely gene expressing vectors to prepare a plasmid, with which a Nef protein is mass-produced as the product of expression of a nef gene.