| 摘要 |
A method of determining the amount of test analyte in a sample using internal calibration comprises: (a) mixing a sample with a predetermined amount of a calibrator analyte foreign to the sample and with a comparable behaviour in an assay to that of the test analyte; (b) contacting the mixture (a) with a solid support containing, each in a separate area, a reagent for binding the test and calibrator analytes, respectively; (c) contacting the solid support with a mixture of labelled reagents for binding the test and calibrator analytes, respectively, and (d) determining the amount of test analyte in the sample by comparing the levels of labelled reagent bound to the test and calibrator analytes. |
