MARKER FOR YEAST STRAIN IDENTIFICATION

摘要

The present invention provides a novel plasmid comprising a beta -glucuronidase (GUS) coding region operably linked to yeast promoter and terminator sequences, whereby said coding region is expressible in yeast. Suitable promoter and terminator sequences are any which allow expression in yeast and are, in particular, Saccharomyces cerevisiae alcohol dehydrogenase promoter and terminator sequences. The plasmid of the present invention is produced by ligating the promoter and terminator sequences to the GUS gene, and inserting the resultant novel construct into an appropriate carrier plasmid. The carrier plasmid is used to introduce the novel construct into a yeast strain by integration into a host chromosome. Assay for GUS activity can then be used to determine the proportion of the marked strain in the total yeast population.