| 摘要 |
<p>Active recombinant human IL-4 is purified from a crude fermentation broth of a secreted E. coli mutant by subjecting said solution to selective chromatography on a zinc-chelating agarose column at pH 7.2 in a buffer containing a high concentration of sodium chloride, i.e., about 1 M sodium chloride, washing first with a phosphate buffer at pH 7.2 containing 1.0 M sodium chloride then with a buffer containing 10 % glycerol and eluting at pH 5.0. The resulting purified active recombinant human IL-4 solution is further treated by cation exchange chromatography, concentration by diafiltration up to 20 mg/ml and size exclusion gel chromatography using a citrate buffer at pH 4.5.</p> |
