| 摘要 |
<p>NEW MATERIAL:A monoclonal antibody capable of recognizing a glycolipid derivative expressed by the formula. USE:A detecting reagent for cancer-related saccharide chain antigens and treating agent for cancer. PREPARATION:For example, the glycolipid derivative expressed by the formula is adsorbed in an emulsion of monophosphoryl-lipid A and dimycolic acid trehalose and repeatedly administered into the abdominal cavity of BALB/c mouse for immunization. After the final immunization, splenic cells are recovered and then fused to mouse myelomatous cells using polyethylene glycol, etc., and a clone capable of producing an antibody specifically binding to the glycolipid derivative expressed by the formula is selected from the produced hybridoma according to the Kohler method, etc., and cloned by a limiting dilution method, etc., to provide a cloned hybridoma. The resultant hybridoma is subsequently cultured to afford the objective monoclonal antibody capable of recognizing the glycolipid derivative expressed by the formula.</p> |
