| 摘要 |
A gene coding for BBRP42, which is a protein secreted in an earlier stage of incubation, is cloned from a genomic DNA library of Bacillus brevis to construct an expression vector containing a signal peptide coding region and a transcription controlling region. By incubating a host carrying a recombinant plasmid containing a foreign gene joined immediately downstream of the signal peptide coding region, it is possible to allow a foreign gene product to be secreted from the host in an earlier stage of the incubation where a protease is not secreted so much, thus enabling an efficient recovery of the product. <IMAGE> |
