| 摘要 |
PURPOSE:To prevent the decrease in detecting sensitivity with the elapse of time and to obtain stable detecting performance for a long period by forming a protecting film on the surface of a counter electrode. CONSTITUTION:The patterns of a measuring electrode 2 and a counter electrode 3 are formed on the surface of a ceramic substrate 1. A ground film 4, an interference removing film 5 and an enzyme immobilized film 6 are sequentially formed on the measuring film electrode 2. The surface of the film 6 is exposed. A protecting film 7 is provided on the counter electrode 3. The protecting film 7 is formed by applying the aqueous solution wherein bridging agent is added into macromolecular material such as gelatin, albumin and polyallylamine on the surface of the counter electrode 3 and performing bridging reaction. In this way, attachment of impurities such as protein on the surface of the counter electrode 3 is prevented when the current and the potential difference between the measuring electrode 2 and the counter electrode 3 are measured and the material to be measured is detected. The decrease in sensitivity of the enzyme immobilized electrode 6 with the elapse of time is prevented, and the stable detecting performance can be displayed for a long period. |
