| 摘要 |
PURPOSE:To improve productivity by culturing a mold in a culture medium containing NaCl and phosphoric acid at prescribed concentrations. CONSTITUTION:With 100-900mM NaCl, are blended 100-900mM phosphate such as K3PO4 or KH2PO4, glucose, peptone, various vitamins, etc., to provide a liquid culture medium (A). A mold (B) such as Aspergillus.oryzae IFO 4348 is then inoculated into the culture medium (A) and cultured at 25-40 deg.C, pH 3-8 and 0.1-2 (vol./vol./min) aeration rate for 2-5 days by aeration, shaking or spinner culture to afford a culture (C), which is then centrifuged to provide a liquid phase part. The resultant liquid phase part is subsequently purified by ion exchange chromatography, etc., to produce a highly active protease. |
