| 摘要 |
PURPOSE:To enable obtaining of a treating and preventive agent useful for thrombosis, etc., by culturing tempeh or tempeh fungus in a proliferative culture medium. CONSTITUTION:Tempeh or tempeh fungus is extracted with an aqueous medium or cultured on a fibrin plate culture medium, etc., to provide an extract or culture (A). The resultant ingredient (A) is then kept at a desired temperature for a desired time, then concentrated, dialyzed and treated by ion exchange chromatography, etc., to afford a crude purified substance (B). The obtained ingredient (B) is subsequently purified by an isoelectric focusing method, etc., to produce a thrombolytic enzyme having characteristics of molecular weight; 20000 to 50000 (a zymographic method); properties; white powder; thermal stability; stable even when kept at pH7.4 and 50 deg.C for 10min; substrate specificity; strongly decomposing fibrin and having a plasminogen activating ability with a powerful hydrolyzing activity against synthetic substrate compounds, etc., expressed by the formula; influence of inhibitor; strongly inhibited with diisopropyl fluorophosphate, etc. |
