Primers and probes for nucleic acid detection.

摘要

The invention provides an oligonucleotide primer exhibiting complementarity to an end of a nucleic acid sequence corresponding to a second exon sequence in an HLA Class II gene, wherein said primer can be hybridized to said sequence and then extended thereon to produce a single stranded extension product which includes a sequence complementary to said second exon sequence. Such primers can be used in a nucleic acid amplification process to enable detection of gene variations or polymorphism. For detection purposes, the invention also includes an oligonucleotide probe that is capable of hybridizing to a sequence in the second exon of an HLA Class II gene so as to permit detection of said sequence when said probe is labelled as so-hybridized.