METHOD FOR OBTAINING A RECOMBINANT PROTEIN AND ITS UTILIZATION IN THE ASSAY FOR THE AFRICAN SWINE PEST VIRUS (ASPV).

摘要

The method consists of (a) purifying the viral capsid protein p72 by HRLC and obtaining the partial sequence of amino acids TPDDPGAMMITF: (b) identifying the gene which encodes this protein, with a radio-actively tagged oligo-nucleotide probe; (c) determining the complete sequence of the 1938 nucleotides forming the gene; (d) expressing the gene in a bacterial host such as E. coli BL21 (DE3) by means of a plasmid of pAR3038 type; (e) cultivating the bacteria transformed by the recombinant plasmid and purifying the recombinant protein p72 from the extracts; and (f) fixing the purified protein or micro-test plates and incubating with pig serum samples to detect the presence of anti-bodies to ASPV.