发明名称 |
INOSINE GUANOSINE KINASE. |
摘要 |
A new inosine guanosine kinase (IGK) catalyses the synthesis of 5'-inosinic acid from inosine and ATP or from inosine and deoxyadenosine triphosphate (dATP); and the synthesis of 5'-guanylic acid from guanosine and ATP or from guanosine and dATP. The structure of the enzyme is given (454 amino acid residues); its mol.wt. is 48400. The working pH is 6.9-8.2 and working temp. 25-40 deg.C. The enzyme is stable over the range pH 6.6-9.0 for 16 hrs. at 4 deg.C and for 15 min. at 40 deg.C and pH 7.2. It is inhibited by Co2+, Cu2+ or Zn2+ but activated by K+/Mg2+ or K+/Mn2+. Also claimed is a DNA sequence coding for the enzyme; such DNA incorporated in a suitable vector; and transformant organisms (esp. of E. coli) incorporating the vector. |
申请公布号 |
EP0458971(A1) |
申请公布日期 |
1991.12.04 |
申请号 |
EP19900917691 |
申请日期 |
1990.12.04 |
申请人 |
KYOWA HAKKO KOGYO CO., LTD. |
发明人 |
MORI, HIDEO;IIDA, AKIHIRO;TESHIBA, SADAO;FUJIO, TATSURO |
分类号 |
C12N1/21;C12N9/12;C12N15/54;C12P19/32 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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