METHOD OF IDENTIFYING HUMAN LEUKOCYTE TYPES

摘要

A method of judging the type of a human leukocyte antigen (HLA) which is a human histocompatible antigen playing an important role in tissue transplantation and diagnosis of autoimmune diseases and drug sensitivity, said method comprising hybridizing a probe labeled with an acridinium ester through a linker arm under hybridization conditions to the product of amplification of either cDNA prepared from mRNA contained in the specimen or genomic DNA contained in the specimen; decomposing selectively the labeled portion of the unhybridized probe and that of the probe which is hybridized but has mismatching of one or more bases in the crossing; and determining the residual chemiluminescence. This method makes it possible to judge the subtype of the class II gene of HLA, which has been serologically impossible heretofore.