发明名称 |
NUCLEOTIDE SEQUENCE ENCODING A 52 KDA RO/SSA AUTOANTIGEN |
摘要 |
Complementary DNA encoding a 52 kDa form of a protein present in the human Ro/SSA ribonucleoprotein complex has been cloned. A lambda gt11 cDNA library made from human thymocyte mRNA was screened with serum from a SLE patient and two immunoreactive clones were isolated. These clones reacted with other patient sera which had anti-52 kDa Ro/SSA antibodies and with affinity purified anti-52 kDa Ro/SSA antibodies. Moreover, affinity purified antibodies eluted from fusion proteins of the isolated clones reacted only with the 52 kDa protein of lymphocytes in the Western blot. Ro/SSA RNAs were also precipitated with these affinity purified antibodies, further confirming that the clones encode a 52 kDa Ro/SSA antigen. The sequence differs from the previously reported 60 kDa Ro/SSA gene. |
申请公布号 |
AU7958891(A) |
申请公布日期 |
1991.11.27 |
申请号 |
AU19910079588 |
申请日期 |
1991.05.07 |
申请人 |
OKLAHOMA MEDICAL RESEARCH FOUNDATION |
发明人 |
MARK B FRANK;KAZUKO ITOH |
分类号 |
A61K38/00;C07K14/47;C07K16/18 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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