Method for measurement of alpha -amylase activity

摘要

A for measurement of alpha -amylase activity uses a substrate comprising a malto-oligo saccharide represented by general formula (I) or (V) described below: A - Gn - B (I) A - Gn - I (V) wherein A represents: <IMAGE> (II) or <IMAGE> (III) B represents a monosaccharide or a derivative thereof other than glucose; I represents inositol or a derivative thereof; G represents glucose; and n represents an integer of 3 to 15; in formula (II) or (III), R1 to R4 each represents a hydrogen atom, a lower alkyl group or (CH2)yCOOM (wherein y is 0, 1 or 2 and M represents a hydrogen atom or an alkali metal); and X1 to X4 each represents an oxygen atom or a sulfur atom. The method for measurement of alpha -amylase activity comprises contacting a substrate containing a malto-oligo saccharide represented by general formula (I) or (V) described above with a sample in the presence of glucosidase and measuring a liberated monosaccharide, inositol or a derivative thereof thereby to measure alpha -amylase activity in the sample.