发明名称 |
PROTEIN PURIFICATION METHOD |
摘要 |
PATENT CL-185 Method of purifying a protein from a solution of substances comprising the steps of passing the solution through an ion exchange material at a pH which facilitates binding of the protein of interest, washing the bound protein at a different pH at which the bound protein does not elute but at which the free protein would not bind to the ion exchange material, and then eluting the protein at a pH which facilitates elution. Method is especially useful in purification of antibodies such as antibodies to tumor necrosis factor.
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申请公布号 |
CA2042945(A1) |
申请公布日期 |
1991.11.26 |
申请号 |
CA19912042945 |
申请日期 |
1991.05.21 |
申请人 |
MILES INC. |
发明人 |
COAN, MICHAEL H.;LEE, VIVIAN W. |
分类号 |
C07K14/52;C07K1/00;C07K1/18;C07K14/525;C07K16/00;C07K16/06;C07K16/24;(IPC1-7):C12P21/08;A61K39/395 |
主分类号 |
C07K14/52 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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