PARTICLE-MEDIATED TRANSFORMATION OF WOODY PLANT SPECIES

摘要

2064017 9118094 PCTABS00008 The creation of transgenic trees is disclosed through the use of a particle-mediated transformation procedure. The method is demonstrated to be effective with various tissue types of poplar, including protoplasts, internode and petiole segments, and, most efficiently, with nodule cultures. Transgenic trees were recovered which express sufficient levels of the insect specific toxin from Bacillus thuringiensis so as to provide significant toxicity to feeding insects.