VEKTOR UND VERFAHREN ZUR ERREICHUNG EINER HOHEN EXPRESSION IN ZELLEN.

摘要

A DNA construct for fabricating a vector for transfecting a cell to achieve high level expression of a gene within the vector encoding a protein prod. comprises (a) DNA defining an enhancer element operative to enhance promotion of transcription of genes located in regions on both the 5' and 3' sides, (b) DNA defining a transcriptionally competent marker region comprising a promoter and a gene encoding a selectable marker, located in a region on one side of the enhancer element, (c) DNA oefining a restriction site, disposed adjacent the 3' end of a promoter, for ligating a gene encoding a protein prod., located in a region on the other side of the enhancer element at a distance to permit enhancement of expression of the gene encoding a protein prod. and (d) DNA defining a blocking element, interposed between the enhancer element and the marker region, operable to block enhancement of transcription of the gene encoding a selectable marker by the enhancer element.