ASSAY FOR SPECIFIC NUCLEIC ACID SEQUENCES

摘要

An assay method in which a target nucleic acid sequence is detected using a labelled probe comprising a complementary nucleic acid sequence under conditions where the target and probe hybridise and a nuclease requiring double stranded DNA for activity degrades the probe faster than the target. A cycling effect leads to large amount of degraded probe being formed, relative to the amount of target, providing an amplification of detectable degradation products. Also described are new oligonucleotides and kits.