发明名称 METHOD FOR INTRODUCING AND INCORPORATING FOREIGN MATERIAL IN ORGANISM OR CELL AND APPARATUS THEREFOR
摘要 PURPOSE:To easily and stably introduce a foreign material into an organism, etc., by ejecting compressed gas and fine particles carrying foreign material from the tip end of a specific nozzle. CONSTITUTION:The nozzle 1 has a nozzle tip hole 4 having an inner diameter of 1.0mm and a length of 5mm and connected to a hole 2 for holding fine particles carrying foreign material. The hole 2 has an inner diameter of 5mm and a length of 18mm. Foreign material-carrying fine particles are produced by supporting about 1mug of a foreign material such as chlorella gene on about 10mg of tungsten fine particles having particle diameter of about 0.6mum and the particles are placed at the center of the carrying hole 2. The action time of an electromagnetic valve is set to about 0.001sec with a controller 13 built in the valve, the controller 13 is actuated to operate an automatic gun 21 for airless spray-coating and compressed air of about 100kgf/cm<2> pressure is supplied to eject the fine particles carrying the gene for about 0.005sec at a high speed from the tip end of a nozzle 23 controlled by the controller 13. The ejected fine particles are introduced into and taken by the cell 24 of a microalgae, etc., at about 40 deg.C.
申请公布号 JPH03251187(A) 申请公布日期 1991.11.08
申请号 JP19900045798 申请日期 1990.02.28
申请人 KANSAI PAINT CO LTD 发明人 MIURA YUKI;MATSUNAGA TADASHI;KIKUTA MASATO;ICHIKAWA MASAYOSHI
分类号 C12N15/09;C12M1/00 主分类号 C12N15/09
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