发明名称 |
METHOD FOR DETERMINING UNENTRAPPED IONIZABLE BIOACTIVE AGENTS IN LIPOSOME PREPARATIONS |
摘要 |
A method for encapsulation of antineoplastic agents in liposomes is provided, having preferably a high drug:lipid ratio. Liposomes may be made by a process that loads the drug by an active mechanism using a transmembrane ion gradient, preferably a transmembrane pH gradient. Using this technique, trapping efficiencies approach 100%, and liposomes may be loaded with drug immediately prior to use, eliminating stability problems related to drug retention in the liposomes. Drug:lipid ratios employed are about 3-80 fold higher than for traditional liposome preparations, and the release rate of the drug from the liposomes is reduced. An assay method to determine free antineoplastic agents in a liposome preparation is also disclosed. |
申请公布号 |
AU8141791(A) |
申请公布日期 |
1991.11.07 |
申请号 |
AU19910081417 |
申请日期 |
1991.07.29 |
申请人 |
THE LIPOSOME COMPANY, INC. |
发明人 |
MARCEL B. BALLY;PIETER R. CULLIS;LAWRENCE MAYER;GEORGE N. MITILENES;RICHARD S. GINSBERG |
分类号 |
G01N33/544;A61K9/127;A61K31/505;A61K31/65;A61K31/70;A61K33/24;A61K45/08;A61K47/02;A61K47/12;A61P35/00;B01J13/02 |
主分类号 |
G01N33/544 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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